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Crucial for protein metabolism, Pyridoxine impacts brain development and function
Immunoblot assays Tissue samples were crushed on dry ice to obtain homogenous aliquots and then lysed in RIPA buffer (Thermo Scientific #89900) containing Halt protease & phosphatase inhibitor (Thermo Scientific #1861280)
May not be tolerable enough for widespread use
The 3.48% reduction in apoptotic cells did not reach statistical significance, though the molecular markers (caspase-3, PARP, Bcl-2/Bax) were all significant
A separate target trial emulation study published in Blood Advances confirmed this finding in an even larger population
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