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Its protonated epsilon-amino group carries positive charge that affects solubility, chromatographic retention and interaction with polyanionic matrix components, and in a physiological setting the lysine face is implicated in ternary complex formation with serum albumin, which has its own high-affinity N-terminal copper site

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Acidic extracellular pH induces vascular endothelial growth factor (VEGF) in human glioblastoma cells via ERK1/2 MAPK signaling pathway: mechanism of low pH-induced VEGF [published correction appears in J biol chem 2002 may 24;277(21):19242]

Subsequently, the medium was replaced with OPTI-MEM supplemented with 5% FBS and 1% sodium pyruvate