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When monoglutamate folates are taken up into cells, particularly in the liver and kidney where they can be stored (but also in other cells), they are polyglutamated through the action of the enzyme folylpolyglutamate synthase
Following these steps will help you use them safely and effectively
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Polyvinylidene fluoride (PVDF) membrane and other electrophoresis supplies were from Bio-Rad (Hercules, CA, USA)
Multi-compound blends require more attention than single-ingredient products
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