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Treg cells not only interacted with microglia (75), but also regulated the polarization transformation of microglia from M1 to M2 type (80, 81)
They observed that this peptide bound to GLP-1 receptors in pancreatic beta cells, stimulating insulin secretion in a glucose-dependent mannermeaning insulin release occurred only when blood glucose levels were elevated
After centrifugation and thorough removal of the supernatant, the pellets were incubated for 1 h in 60 l staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 l anti-BrdUFITC monoclonal antibody (BioLegend, 364104, clone 3D4)
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