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Transiently transfected HEK 293T cells showed that the C-terminus-truncated GLP-2R(1490)-LgBiT had a 6-fold stronger interaction with G-SmBiT than the wild-type (WT) receptor(1553)-LgBiT (Supplementary information, Fig
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Cells from a 50 mL overnight culture in LB medium were harvested by centrifugation (4,000 g, 10 min) and resuspended in 20 mL MOPS minimal medium ( 578 ~ 0.2) containing 50 g mL 1 ampicillin (pGEX-6P-1- cntAB - bcct ), 17 g mL 1 chloramphenicol (pACYCDuet-1- msadh - mdh ) and 500 M IPTG
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