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The cells were lysed with RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Calbiochem, 524-624 and 524-625) and subsequently subjected to western blotting using the indicated antibodies as described previously 16

Therapeutic advantages and challenges of targeting central GLP-1 The potential therapeutic benefits of targeting the brains GLP-1 system are significant

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