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Hind limb blood flow measurement with laser speckle imaging To assess endothelium-dependent vasodilation in the quadriceps muscle, five animals per group (WT and VECAD IGF- 1R KD) were anesthetized using isoflurane (induction 34%, maintenance 1.52%) in oxygen and mechanically ventilated to ensure stable respiration

10.2337/diabetes.52.12.2874 43 SaiharaK.KamikuboR.IkemotoK.UchidaK.AkagawaM

Timing: Take on an empty stomach or with a small amount of healthy fat to optimize cellular uptake

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Eur J Pharm