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Gene expression analysis Total RNA was extracted from samples using RNeasy Mini Kit (Qiagen, Hilden, Germany) and used for complementary DNA synthesis using the ReverTra Ace quantitative RT-PCR Kit (Toyobo, Osaka, Japan)

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In contrast to the heme of hemoglobin and myoglobin, the heme iron of all cytochromes participate in the cyclic redox reactions of electron transport, alternating between the oxidized (Fe 3+ ) and reduced (Fe 2+ ) forms

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This includes determining the appropriate dosage, administration method, and monitoring protocols