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Initially described as a mediator of exercise-induced browning of white adipose tissue and increased energy expenditure, irisin was proposed as a candidate exercise- associated signaling molecule ( Subsequent debate regarding assay specificity, circulating concentrations, and translational relevance led to skepticism regarding irisins role in humans
Instead, it carries something called a beyond-use date, or BUD
(January 1973)
In this review, examples will be discussed how experimental conditions (dose, solvent, etc.), the experimental system (species, strain, and substrain in vivo, cell type, and in vitro conditions), and also adaptive responses and off-target effects of genetic manipulations and chemical interventions, can impact the mechanisms of cell death
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