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Brains were fixed, cryoprotected, and sectioned for immunofluorescent labeling using anti-Fos antibodies and fluorescent secondary antibodies

2Inject the water slowly down the inside wall of the peptide vial, avoiding foaming or vigorous agitation

Funding The study was supported by the Henan Province Science and Technology Project (182102310112, ZY), the National S&T Major Project of China (2018ZX10301201-008), National Natural Science Foundation of China (81600506), National Key Research and Development Program of China (2018YFC2000501), and China Postdoctoral Science Foundation (2017464 and 20182814)

& von Zglinicki, T

doi: 10.3390/separations11090257 9