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One milliliter of a PBS-based reaction mixture containing propidium iodide (20 g/mL), RNase (0.2 g/mL), and 0.1% Triton X-100 (Sigma) was added to the cell samples at a density of 1 10 6 cells/mL and incubated in the dark for 40 min before a DNA content analysis was conducted through flow cytometry
Miura H, Kusakabe Y, Harada S
Ease of use and directions
Swirl, never shake and add water down the glass wall
"This lets melanotan trick the body into darkening the skin without that [UV] trigger," she told ScienceAlert
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