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To label these chimeric receptors at their C-termini with either NLuc or RLuc8, the plasmid was digested with HindIII and XbaI, and the purified fragment was ligated into either Arr3-NLuc or the GLP-1 receptor labeled at the C-terminus with RLuc8 (GLP-1RRLuc8) that had previously been digested with HindIII and XbaI, replacing the Arr3 or GLP-1R open reading frame with that of the chimeric receptor

After 72 h treatments, 0.5 g ml 1 propidium iodide (Sigma-Aldrich, P4864) and 1.4 g ml 1 Hoechst 33342 (Invitrogen, H3570) were added to cells

These findings help explain why copper peptides became established cosmetic-research ingredients

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