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To induce osteogenic differentiation, growth medium was aspirated and replaced by osteogenic medium (-MEM supplemented with 10% FCS, 10 U/mL penicillin, 10 g/mL streptomycin, 10 mM -glycerophosphate, 200 M phosphoascorbic acid, 2 mM CaCl 2 , and 0.2 M dexamethasone), in the absence or presence of increasing concentrations of PAMPs or 10% (v/v) BMDM-conditioned media following PAMP stimulation as described above
Another patient with FTO rs9939609 risk alleles and lower natural carnitine transporter activity might benefit significantly from targeted L-carnitine support
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