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Gli1 SuSC are activated and migrate after injury Cxcl12-Cxcr4 signaling guides migration and expansion Shh/Ihh are secondary niche signals promoting differentiation Gs-Gi balance regulates niche signaling This study provides a foundation for therapeutic strategies targeting Cxcl12-Hh-Gs signaling to enhance bone repair or treat craniosynostosis and related diseases

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