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Ethanol and detergents like SDS and Triton X-100 are good at removing proteins that are hydrophobically bound to the column
Western Blot Total proteins were extracted in RIPA buffer (Sigma-Aldrich) that was supplemented with protease inhibitor cocktail (100 Sigma-Aldrich)
Discovery work characterised it as roughly 0.4 as active as native GLP-1, 0.3 as active as native glucagon and about 8.9 as active at the GIP receptor [6]
268 When activated, the vagus nerve can reduce the contraction of the stomach, thereby slowing down food emptying
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