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For all sites: you are injecting into the subcutaneous fat layer
Slices were then transferred to wells filled with 600 L of ACSF ( + 100 mM KCl) with DPP4 inhibitors (24 L/600 L) and Aprotinin (9 L/600 L) and incubated for 20 min at 34 C (monitoring of bubbling, keeping slices in motion using a pipette)
5), S42S47 (2001)
If you want to understand more about why injection-site marks happen, our guide on why semaglutide injection sites can bruise explains the mechanics in more detail
This could be genetic, due to stress, nutritional deficiencies or certain health conditions
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