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This peroxidation occurs via two major pathways: the enzymatic pathway, mediated by lipoxygenases (LOXs), cytochrome P450 oxidoreductase (POR), and NADPH oxidases (NOXs), promotes the generation of PUFA-PL-OOHs, leading to the accumulation of reactive aldehydes, ketones, and other toxic metabolites, ultimately disrupting membrane integrity and triggering ferroptosis (Maheshwari, 2023)

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Cells were incubated with10 M dichlorodihydrofluorescein diacetate and ROS accumulation was estimated from MFI by FCM