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FIGURE 1 Iron metabolism Iron can generate ROS through enzymatic or non-enzymatic reactions

In this study, we have revealed the importance of the glutamate-generating metabolism, as shown by a complete depletion of glutamine (Gln) in the medium during H

doi:10.1007/s00535-013-0869-z 55

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However, it can also be useful in combination with other triggers, such as hypoxia, because studying multiple triggers provides a more holistic understanding of the mechanisms driving ECD and allows for a more robust and representative model that mirrors the pathophysiological conditions observed in vivo [219]