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Madine-Darby canine kidney (MDCK) epithelial cells were maintained in Eagles Minimum Essential Medium (EMEM) media (Sigma Aldrich) supplemented with 10% FBS, 5% penicillinstreptomycin and amphotericin B

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Finally, Drosophila do not have an adaptive immune system, which complicates both the study of tissue restitution after injury and the underlying crosstalk with intestinal microbes