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Although many studies with glutathione have not shown serious adverse reactions, mild side effects have been documented with glutathione taken orally including: Because supplements such as glutathione are not regulated as rigorously as drugs by the FDA, the quality of products sold as supplements can be difficult to verify

GSH is synthesized in two steps: First, gamma-glutamylcysteine (-GC) is formed from glutamate and cysteine, which is the rate-limiting step in GSH production

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The mitochondrial precipitate was stored in a resuspension medium containing (mmol/l): sucrose250 and Tris-HCl25 (pH 7.2), and immediately used in the experiment or stored at low temperatures

doi: 10.1016/j.taap.2018.12.001 150 ZhuHWuZYuYChangKZhaoCHuangZet al