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For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)
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About this article Cite this article Nagaraju, S., Ahmed, S.S., Raghunathnaidu, B.D
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