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Detached cells were suspended in the modified DMEM medium and seeded at 10 4 cells/well in 96-well microtiter plates (Costar, Corning) and incubated in the presence of 5% CO 2 at 37 C for 24 h
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Because the membranes of reproductive cells are particularly rich in these fatty acids, they are highly susceptible to this type of damage
Gentle swirling or rolling the vial between palms suffices
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