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Detached cells were suspended in the modified DMEM medium and seeded at 10 4 cells/well in 96-well microtiter plates (Costar, Corning) and incubated in the presence of 5% CO 2 at 37 C for 24 h
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Because the membranes of reproductive cells are particularly rich in these fatty acids, they are highly susceptible to this type of damage
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