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Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution
The injection of bee venom has been reported to evoke hyperalgesia and a sharp pricking sensation followed by tonic pain lasting for a few minutes up to 12 h
S., Christensen, S
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