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In Vitro Skin Irritation Test HaCaT keratinocytes were cultured in DMEM containing 10% fetal bovine serum and 1% penicillin/streptomycin and were maintained under 37 C with humidified 5% CO 2

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One study found that 32% of patients receiving IV glutathione experienced adverse events, including liver dysfunction and one case of anaphylaxis.[8] The FDA has issued warnings about contaminated IV glutathione causing hospitalizations.[9] Theres no long-term safety data for ongoing IV use

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