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Dihydrothymine is then converted to N -carbamoyl-BAIBA via the action of dihydropyrimidase (encoded by the DPYS gene)
The primary inoculum (1 mL) was transferred into 140 mL of fresh LB medium in a 500 mL flask and cultured for 8 h to obtain the secondary inoculum, which was inoculated directly into the bioreactor
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