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The first and limiting step is the conjugation of cysteine with glutamate to form the dipeptide -glutamylcysteine, which is catalyzed by -glutamyl-cysteine ligase (GCL)
Tiny injections are placed directly into muscle tissue
V.DonatoM
Blood 98:280b, 2001
JA and JA-Ile quantification in leaf tissues Metabolite extraction and quantification of JA and JA-Ile in leaf tissues were performed according to previously described methods using AB Sciex 4500 QTRAP triple quadrupole mass spectrometer (AB SCIEX, MA, USA) equipped with an ACQUITY UPLC BEH C18 column (Waters, Eschborn, Germany) (50 2.1 mm, 1.7 m) 45
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