The concurrent engagement of all three receptors is hypothesised to produce complementary metabolic effects in pre-clinical models: GLP-1R activation is associated with glucose regulation and appetite modulation, GIPR activation with potentiation of GLP-1mediated responses, and GcgR activation with energy expenditure and hepatic lipid metabolism in laboratory settings
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GLP-1 Receptor Agonists and Ocular Outcomes: Metabolic Transition, Retinal Vulnerability, and Risk-Stratified Monitoring
Immunofluorescence imaging of cells and pancreatic slices Dispersed islet cells of healthy humans ( n = 3) or mice ( n = 5) were seeded on coverslips coated with 5 g/mL rhLaminin-521 (Gibco, catalog A29249) and incubated (24 hours) in RPMI 1640 supplemented by 11 mM glucose, 10% FCS, 10 mM HEPES, and 1% penicillin-streptomycin in cell culture incubator
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