purification of proteins fused to glutathione s-transferase Glutathione-S-Transferases as Potential Targets for Modulation Nitric Oxide-Mediated Vasodilation In-house preparation of hydrogels for

hydroxycobalamin) is not itself a coenzyme form of B12, yet can easily be converted by the body into both methyl- and adenosylcobalamin, and is a common transitional form in the B12 metabolism

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On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler

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