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Research chemical manufacturers typically use solid-phase peptide synthesis, which can introduce truncated sequences, deletion peptides, racemized amino acids, and residual coupling reagents as impurities

In the D-Gal + FOXO4-DRI group, mice also received D-galactose injections and, after 4 weeks, were given intraperitoneal injections of FOXO4-DRI (5 mg/kg, MCE, HY-P4157) every 2 days for 4 weeks, followed by tissue collection

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This process may take several months, as dose changes require time to stabilize in the body and for symptoms to improve