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Cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS, L-glutamine, penicillin, and streptomycin at 37 C in 5% CO 2
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Because DSIP is studied in the context of sleep architecture normalization, stress response modulation, and HPA axis regulation, it is a subject in experiments related to sleep-stress interactions and emotional well-beingwithout implying outcomes outside of a research setting.
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