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Cells were permeabilized in 0.2% (v/v) Triton X-100 for 5 min, blocked in 1% bovine serum albumin and 2% donkey serum for 30 min., incubated with primary antibodies for 1 h, washed with PBS and finally incubated with FITC- or Cy3- conjugated secondary antibodies (Jackson ImmunoResearch)

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Reagent II: 20 mL1

Elevated serum melatonin is associated with the nocturnal worsening of asthma