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The beads were suspended in 500 l of 25 mM ammonium bicarbonate buffer containing 5 mM dithiothreitol (DTT) and incubated at 65 C for 20 min

In a study in Israel, the odds ratio (OR) of ASD in offspring marginally increased for paternal ages of 3039 years and significantly increased (OR = 5.75) for paternal ages of 40 years and older when adjusted for maternal age and compared with paternal ages of 3.0.CO;2-O 32

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All discussion is limited to published or controlled in vitro assays and laboratory animal model research conducted under institutional oversight