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With advancing age, several factors may alter glutamine kinetics, including reduced muscle mass, impaired protein turnover, and increased inflammatory signaling

Several different targeting strategies for the insulin and IGFI receptor family exist, and dozens of drug candidates have shown activity in model systems

doi: 10.1016/j.bioorg.2020.103792 171 AlkafaasSSLoutfySADiabTHessienM

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Tissues were incubated in a 15 ml Falcon tube with 1 ml of collagenase P solution for 15 min at 37 C, followed by the addition of 12 ml of cold G-solution (Sigma-Aldrich) and centrifugation at 586 g at room temperature