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Figure 5 Contrary to the results obtained after modification in the C-terminal of Ang IV where essentially all compounds examined exhibited some activity, oxidative cyclization of the side chains of Cys 1 and Cys 3 and consequently creation of an 11-membered disulfide macrocycle in the N-terminal rendered the inactive compound 20 (Figure 6)

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Amplified products were visualized on a 1.5% agarose gel and purified using a kit (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel, Dren, Germany) according to manufacturers instructions, for subsequent Sanger sequencing

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