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The 2025 systematic review noted that despite increasing clinical use, this level of evidence does not yet exist
Genomic DNA was then isolated with the help of NucleoSpin Tissue XS from the clonal cell lines and subjected to sequencing to identify frameshift mutations by amplifying the genomic regions with the following primers: for ARL8A KO, forward 5- GATTCCGGGGCAGCGAGTCGT-3 and reverse 5-TACTAGGCCCCAAGCGCTCG-3, and for ARL8B KO, forward 5-TCATCTCCCGCCTGCTGGACT-3 and reverse 5-TCCAAATGCTGGCCCTCGG-3
doi: 10.1053/j.jrn.2022.01.015 176 YanQ.LiuM.XieY.LinY.FuP.PuY.et al
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