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For viability, 7-aminoactinomycin D (7AAD) (Thermo fisher A1310) or Zombie NIR (BioLegend 423106) diluted 1:3000 was stained concurrently with antibodies in all flow cytometry assays
However, despite the small amount needed, the effects of a deficiency can be devastating
So even if you eat glutathione-rich foods or take supplements, only a small fraction of the amount affects your actual levels
B., Ertl, N., Phylactou, M., Muzi, B., Patel, B., Alexander, E
Some protocols use cycling (a few weeks on, then off)
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