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The thermocycler protocol involved an initial denaturation cycle (5 min, 95 C), 40 cycles of denaturation (45 s, 95 C), annealing (30 s, 5658 C), and extension (30 s, 72 C), followed by a final extension cycle (5 min, 72 C) terminating at 4 C
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Because IV therapy lacks FDA approval for skin lightening, and the long-term effects remain unknown, it is crucial to weigh the effectiveness against these potential side effects
At body temperature cystine which is released from protein diet interacts with allicin in gastrointestinal tract (GI) tract to generate two S-allylmercaptocysteine (SAMC) derivatives (113)
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