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Aliquoting Protocol Decide on a volume per aliquot based on your typical experiment size (e.g., 50100 L per aliquot) Use low-binding microcentrifuge tubes (protein low-bind or equivalent) Label each tube: compound name, concentration, date, lot number Flash freeze aliquots in liquid nitrogen or dry ice/ethanol bath before transferring to -80C storage Discard any aliquot that has been thawed and refrozen more than once See: Optimal Storage and Stability Guidelines for IGF-1 LR3 Lyophilized Research Peptide for detailed post-reconstitution storage guidance

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These effectors reduce the affinity of the enzyme for pyruvate, thus limiting the flow of carbon through the PDH complex