At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S -transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta
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At the end of the experiment hECs were lysed in DW supplemented with Triton X-100 detergent (10 mg/ml)
I spent months doing computational modeling [to design peptides with amino acid structures that would engage with the receptors] within a narrow category as I couldnt choose a molecule that had previously been tested in a clinical context [because it could not then be legally marketed as a dietary supplement]
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