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Reverse transcription and real-time quantitative PCR (Q-PCR) Total RNA was isolated from frozen liver pieces using Tri-Reagent (Molecular Research Center, Inc.) according to manufacturers protocol

Since GLP-1 secretion is physiologically triggered by nutrient intake or glucose exposure, we anticipated minimal interference from Exe4

Most previous work on biogenic INPs has focused on complex mixtures of organic matter, often composed of both whole microorganisms and non-living organic matter 19,34,35,36