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Ginsenosides, the primary bioactive compounds in Panax ginseng , exert potent anti-inflammatory effects by suppressing proinflammatory cytokines and inhibiting signaling pathways such as NF-B

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Higher plant APR are homodimers with each monomer formed by an N-terminal domain binding a [4Fe-4S] cluster and bearing the APR activity and a C-terminal GRX-related domain (Figure 2 and Supplementary Table 2)

Mass Spectrometry (MS): This analytical step validates the identity of the compound by measuring its precise molecular mass, verifying that the actual sequence matches the true delta sleep-inducing peptide chain