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For the open field test, experimental animals need to be screened

Depending upon cell type BH 4 can be generated by several different (alternative) de novo biosynthesis pathways as well as by two different salvage pathways

a multi-class reactive oxygen species (ROS) panel (global ROS, superoxide, hydrogen peroxide, hydroxyl radical)

Isolation of splenic lymphocytes and magnetic bead sorting of CD8 + T cells from normal mice Fresh mouse spleen tissue samples were finely minced in a culture dish and homogenized