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Sphingomyelin-cholesterol interactions in biological and model membranes
However, every freeze-thaw cycle degrades the peptide structure
An FEI Vitrobot Mark IV (Thermo Fisher Scientific) was used to plunge-freeze the grids into liquid ethane after sample application at 4 C and 100% humidity, with a blot time of 3 s and a blot force of 5
The lipid extracts were cleared by centrifugation (17,000 g , 20 C, 10 min), and the lipids in the supernatant were analyzed by LC-MS
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