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Potential body composition effects Growth hormone plays established roles in both lipolysis and muscle protein synthesis

Studies show that most patients regain a significant portion of lost weight within months of stopping treatment as appetite returns to pre-treatment levels and metabolic signaling reverts

10.1007/s11920-018-0977-9 71

Current opinion in pharmacology, 7(4), 355359

Cells were washed once in 1.25 M glycine/PBS and lysed for 60 min on ice with homogenization in 1 mL RIPA buffer (50 mM Tris HCl, pH 8.0, 150 mM sodium chloride, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, protease inhibitors (Complete mini, EDTA-free, Roche))