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10.1038/gt.2008.68 Gene
The PCR conditions consisted of denaturation at 95 C for 3 min, followed by 40 cycles of denaturation at 95 C for 30 s, annealing at 58 C for 30 s and extension at 72 C for 30 s
[63] [39] A human fetus appears to be at triple risk from maternal alcohol consumption: [64] [65] The placenta allows free entry of ethanol and toxic metabolites like acetaldehyde into the fetal compartment
This concentration halves the injection volume compared to 2.5 mg/mL
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