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This heterogenous and complex disease encompasses a broad clinical spectrum of liver abnormalities which vary in the severity of the injury and the resulting fibrosis

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For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C

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Beyond its crucial role as a primary glutathione precursor, NAC possesses its own potent, direct antioxidant capabilities that function independently of the glutathione pathway