Weight should be monitored regularly for all patients, along with vital signs including blood pressure and heart rate
Support healing with nutrients like zinc, glutamine, butyrate, NAC, glycine, and vitamin C
Preparation of the muscle specimens and frozen section To prepare frozen muscle sections, we first anesthetized adult fish with a 0.025% buffered MS-222 solution (Sigma-Aldrich, US), and then removed a segment of muscle tissue located between the anal and caudal fins 35
In practice, bacteriostatic water is used when multiple injections or withdrawals will be needed (thanks to its preservative), whereas sterile water is used when only one-time immediate use is required
38 This proposes an interesting question