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Extracts were clarified by centrifugation and filtration through a 0.45 m pore Spin-X filter (Corning), and the total protein concentration was determined by BCA assay

Sun Z, Li S, Yu Y et al (2018) Alterations in epididymal proteomics and antioxidant activity of mice exposed to fluoride

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The pituitary gland could become less responsive to the ipamorelin signal, requiring higher doses for the same effect

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