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Sample volumes were normalized to 35 L with EPPS buffer (200 mM, pH 8), diluted with HPLC grade CH 3 CN (9 L), and then labeled (5 L of 20 g/L per sample) with the corresponding TMTsixplex Isobaric Mass Tag (Themo Scientific, #90064B)
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However, the chemical composition of GSH does not allow it to be taken orally for central nervous system diseases because little to none crosses the blood-brain barrier
promotes anti-cancerous properties inside the body
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