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Due to both the high abundance of GSH and the millimolar K d value, we observed F 405 /F 488 changes only when RT reacted with GSH but not other thiols, reactive oxygen or nitrogen species (ROS/RNS) at their corresponding physiological concentrations (Fig

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Melatonin administration reversed this phenotype by adopting a compensatory mechanism aimed at increasing Bcl-2 protein expression, blocking Bax proapoptotic activity via the SIRT1/NF-kB axis with a consequent and significant inhibition of Cyt C release and the lack of apoptosome formation and caspase 3 activation 37 (Fig

294 , R1618R1627 (2008)